John was one of many children of John Jackson (1712-1791) and Sarah Miller.

He married Susanah Jackson (no relation), a descendant of Ephraim Jackson (1658-1731) of Edgmont, Pennsylvania, in 1768 at the New Garden Quaker Monthly Meeting in Chester County, Pennsylvania. This family left PA in 1783-85 to settle at Beaver Harbour in Charlotte County, Nova Scotia (now New Brunswick), but returned to the Delaware/Maryland area in 1788 after the birth of their then youngest son, Thomas. Until 1795, the family were connected to the Gunpowder MM in Baltimore County, Maryland, where John died in 1795. The remains of the family were then associated with the Wilmington, Delaware MM, then with the Kennett, Pennsylvania MM by December 1799.

Their son Thomas probably married between 1808 and 1814, and was probably NOT the father of John C. Jackson (1815-1896), born in Chester County, Pennsylvania in about 1815, of parents who were born in Canada and possibly were at least of part-Potawotami heritage..